Research on the stability of concentration and biological activity of preparation including Bacillus subtilis, quercetin under experimental conditions
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https://doi.org/10.54939/1859-1043.j.mst.FEE.2023.264-270Keywords:
Bacillus subtilis; DPPH; Quercetin.Abstract
The efficiency of a biological preparation depends on many factors, especially the density of its bacteria. During shelf life, protective conditions do not have any factor that reduces the number of spore bacteria. This study determined the stability of quercetin content (mg), density of Bacillus subtilis (CFU/g) and DPPH antioxidant activity (%), antibacterial activity, etc., in storage time. The obtained results showed that after storage time at room temperature of 30 °C, the quercetin content in the preparation was maintained at 44,74 ± 0,15 mg/g, the density of Bacillus subtilis was about 5,32 x 106 CFU/g. The product, during storage, showed antioxidant activity with the ability to neutralize DPPH > 60% and was active against tested bacteria E. coli, Salmonella at experimental conditions.
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