Determining the conditions for obtaining Bacillus subtilis biomass to create Synbiotic-Plus

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Authors

  • Nguyen Ha Trung Institute of New Technology, Academy of Military Science and Technology
  • Nghiem Ngoc Hoa Institute of New Technology, Academy of Military Science and Technology
  • Nguyen Lam Anh (Corresponding Author) Institute of New Technology, Academy of Military Science and Technology
  • Le Huy Hoang Institute of New Technology, Academy of Military Science and Technology
  • Pham Kien Cuong Institute of New Technology, Academy of Military Science and Technology

DOI:

https://doi.org/10.54939/1859-1043.j.mst.FEE.2022.253-259

Keywords:

Bacillus subtilis; Synbiotic; Probiotic; Prebiotic.

Abstract

Synbiotics are food supplements that protect the health, they are made up of two main ingredients, fiber (prebiotic) and probiotics (probiotic). Synbiotics have the function of beneficial bacteria, balancing the microbiome in the gut. In Vietnam, Bacillus subtilis is widely used in probiotic production. Bacillus subtilis is of interest because of its spore-forming ability. Spores can germinate and grow under anaerobic conditions in the intestinal tract, thus maintaining a population of beneficial bacteria in the gut. The purpose of this study is to evaluate the influence of medium factors on the growth and development of the strain and determine some properties of the strain, thereby making a premise for fermentation to produce Synbiotic-Plus. The results determined the conditions for culturing Bacillus subtilis strains: the optimal media for the growth and development of B. subtilis strain was DSM, pH 7, 37 °C, speed 200 rpm, 5 days of incubation. Spore formation efficiency was 85,57%. The strain B. subtilis exhibits the properties of the probiotic strain such as the ability to produce extracellular enzymes (cellulase, amylase, protease), antibacterial ability (E. coli, Salmonella).

References

[1]. Đoàn Thị Ngọc Thanh, Phạm Nguyễn Kim Lài, Phạm Thị Thúy Ngoan. “Đặc tính Probiotic và khả năng làm tan huyết khối của chủng vi khuẩn Bacillus subtilis Natto”. Tạp chí Khoa học Trường Đại học Cần Thơ. 56 (1): 104 – 110, (2020). DOI: https://doi.org/10.22144/ctu.jvn.2020.011

[2]. Ngô Tự Thành, Bùi Thị Việt Hà, Vũ Minh Đức, Chu Văn Mẫn. “Nghiên cứu hoạt tính enzym ngoại bào của một số chủng Bacillus mới phân lập và khả năng ứng dụng chúng trong xử lý nước thải”. Tạp chí Khoa học ĐHQGHN, Khoa học Tự nhiên và Công nghệ. 25: 101 – 106, (2009).

[3]. Nguyễn Lân Dũng, Nguyễn Đình Quyến, Phạm Văn Ty. “Một số phương pháp nghiên cứu vi sinh vật”. NXB Khoa học và kĩ thuật, Hà Nội, 27-35, (1989).

[4]. G. Sreekumar1, Soundarajan Krishnan. “Enhanced biomass production study on probiotic Bacillus subtilis SK09 by medium optimization using response surface methodology”. African Journal of Biotechnology. 9 (45): 8078-8084, (2010). DOI: https://doi.org/10.5897/AJB10.1283

[5]. Hoa T. T., Duc, L. H., Isticato R., Baccigalupi L., Ricca E., Van P. H. and Cutting S. M. “The fate and dissemination of Bacillus subtilis spores in a murine model”, Appl. Environ. Microbiol. 67: 3819-3823, (2001). DOI: https://doi.org/10.1128/AEM.67.9.3819-3823.2001

[6]. Huynh, T. H. N. and Nguyen, T. H. “Examining Some Probiotics Activities of Bacillus subtilis natto”, International Journal of Modern Engineering Research. 6 (5): 33 –37, (2016).

[7]. Jianhua Xie, Rijun Zhang, Changjiang Shang and Yaoqi Guo, “Isolation and characterization of a bacteriocin produced by an isolated Bacillus subtilis LFB112 that exhibits antimicrobial activity against domestic animal pathogens”. African Journal of Biotechnology. 8 (20): 5611-5619, (2009).

[8]. Shimizu M. “Purification and characterization of phytase from Bacillus subtilis(natto) N-77”. Biosci. Biotechnol. Biochem. 56 (8): 1266-1269, (1992). DOI: https://doi.org/10.1271/bbb.56.1266

Published

30-12-2022

How to Cite

Nguyễn Hà Trung, Nghiêm Ngọc Hoa, Nguyễn Lâm Anh, Lê Huy Hoàng, and Phạm Kiên Cường. “Determining the Conditions for Obtaining Bacillus Subtilis Biomass to Create Synbiotic-Plus”. Journal of Military Science and Technology, no. FEE, Dec. 2022, pp. 253-9, doi:10.54939/1859-1043.j.mst.FEE.2022.253-259.

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