Screening of polyhydroxyalkanoate (PHA) produced by bacteria isolated from Truong Sa Lon island
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https://doi.org/10.54939/1859-1043.j.mst.80.2022.126-131Keywords:
Biodegradation; Bioplastic; Polyhydroxyankanoate; Halophilic; Truong Sa.Abstract
Polyhydroxyalkanoate (PHA) is a biodegradable plastic produced by microorganisms. PHA is a form of energy and C storage in microbial cells in the conditions of excess C source and lack of one of the compounds N, S, P, etc. Many microorganisms can biosynthesis PHA but halophilic bacteria have some advantages due to cost and energy savings. In this study, the results of isolation and screening of halophilic bacteria from soils, wastes, and water on Truong Sa Lon island are presented. Two strains of 20 strains of halophilic bacteria isolated from Truong Sa Lon island which are capable of biosynthesis of high PHA were identified by sequencing 16S rRNA gene segment. The results showed that strain TSLT14 belonged to the genus Klebsiella and was named Klebsiella sp. TSLT14. TSLS23 strain belongs to the genus Bacillus and was named Bacillus sp. TSLS23.
References
[1]. Ali WS, Zaki NH, “Production of bioplastic by bacteria isolated from lcal soil and organic wastes”. Current Research in Microbiology and Biotechnology, 5(2), pp. 1012-1017, (2017).
[2]. Dilkes-Hoffman LS, Lant PA, Laycock B, Pratt S. “The rate of biodegradation of PHA bioplastics in the marine environment: A meta-study”. Marine Pollution Bulletin 142, pp. 15-24, (2019).
[3]. Koller M. “Production of Hydroxylankanoate (PHA) biopolyesters by extremophiles?” MOJ Polymer Science, I (2), pp. 69-85, (2017).
[4]. Mitra R, Xu T, Xiang H, Han J, “Current developments on polyhydroxyalkanoates synthesis by using halophlies as a promising cell factory”. Microbial Cell Factories 19:86. https://doi.org/10.1186/s12934-020-01342-z, (2020).
[5]. Ratnaningrum D, Saraswaty V, Priatni S, Lisdiyanti P, Purnomo A,
Pudjiraharti S. “Screening of polyhydroxyalkanoates (PHA)-producing bacteria from soil bacteria strains”. IOP Conf. Series: Earth and Environmental Science 277 (2019) 012003 IOP Publishing. DOI:10.1088/1755-1315/277 /1/012003, (2018).
[6]. Saharan BS, Grewal A, Kumar P. “Biotechnological production of polyhydroxyankanoates: a review on trends and latest developments”. Chinese Journal of Biology, Article ID 802984, 18 pages. http://dx.doi.org/10.1155/2014/802984, (2014).
[7]. Trần Hữu Phong, “Nghiên cứu lên men và thu nhận polyhydroxyalkanoates từ vi khuẩn phân lập ở một số vùng đất của Việt Nam”. Luận án Tiến sĩ sinh học. Đại học Quốc gia Hà Nội, (2017).
[8]. Thomas T, Sudesh K, Bazire A, Elain A, Tan HT, Lim H, Bruzaud S. “PHA production and PHA synthases of the Halophilic bacterium Halomonas sp”. SF2003. Bioengineering, 7, 29. DOI:10.3390. www.mdpi.com/journal/bioenginee-ring, (2020).
[9]. Tufail S, Munir S, Jamil N. “Variation analysis of bacterial polyhydroxyalkanoate production using saturated and unsaturated hydrocarbons”. Brazilian Journal of Microbiology, pp. 48, 629 - 636, (2017).
[10]. Winnacker M. “Polyhydroxyalkanoates: Recent advances in their synthesis and application”. European Journal Lipid Science Technology. DOI: 10.1002/ejlst.201900101, (2019).
[11]. Zhang H, Obias V, Gonyer K, Dennis D. “Production of polyhydroxyalkanoates in Succrose-utilizing recombinant Escherichia coli and Klebsiella strains”. Apply Environment Mirobiology 60, pp. 198-1205, (1994).
