Determination of total nitrogen and phosphorus in soil by ion chromatography with conductivity detector following persulfate digestion method
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https://doi.org/10.54939/1859-1043.j.mst.FEE.2022.272-278Keywords:
Total nitrogen; Total phosphorus; Alkaline potassium persulfate; Ion chromatography.Abstract
This study introduces a new method to determine both TP and TN in one process, reducing the analytical cost and time. During this procedure, soil samples were digested in alkaline potassium persulfate (K2S2O8) at 150 °C for two hours in a reactor to convert organic N (including NH3/NH4+) and P species to the highest oxidation states NO3-, PO43-. After that, H2O2 removes the color of the digest, which could influence the efficiency of the analytical process. Finally, ions NO3- and PO43- were separated on the anion-exchange column and quantified by a conductivity detector. According to the spiked samples, which were made by mixing Bovine serum albumin (BSA) with organic nitrogen and triphenyl phosphate containing organic phosphorus with real soil matrix, the recovery of TN and TP was 85% and 97%, respectively. Both TN and TP linear concentration ranges were between 0.50 and 50 µg.mL-1. The results of relative standard deviation and limit of detection for TN were from 4.2%, and 0.31 mg.kg-1; and for TP were from 3.5%, and 0.25 mg.kg-1.
References
[1]. Charles J.Potton. and Jennifer R.Kryskalla, “Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Evaluation of Alkaline Persulfate Digestion as an Alternative to Kjeldahl Digestion for Determination of Total and Dissolved Nitrogen and Phosphorus in Water”, U.S. Geological Survey WaterResources Investigations Report 03–4174, Denver,CO, (2003).
[2]. Hagedorn, Frank & Schleppi, Patrick. “Determination of total dissolved nitrogen by persulfate oxidation”, Journal of Plant Nutrition and Soil Science - J PLANT NUTR SOIL SCI. 163. 81-82, (2000). DOI: https://doi.org/10.1002/(SICI)1522-2624(200002)163:1<81::AID-JPLN81>3.0.CO;2-1
[3]. John D.Pfaff. “Method 300.0: Determination of inorganic anions by ion chromatography”, U.S. EPA, (1993).
[5]. Lakesh K. Sharma et al. "Improving Nitrogen and Phosphorus Efficiency for Optimal Plant Growth and Yield" In New Visions in Plant Science, edited by Özge Çelik. London: IntechOpen, (2017). DOI: https://doi.org/10.5772/intechopen.72214
[6]. Larry Purcell & Andy King, “Total Nitrogen Determination in Plant Material by Persulfate Digestion”, Agronomy Journal - AGRON J. 88, (1996). DOI: https://doi.org/10.2134/agronj1996.00021962008800010023x
[7]. Leghari, S.J., Wahocho, N.A., Laghari, G.M., HafeezLaghari, A., MustafaBhabhan, G., HussainTalpur, K. and Lashari, A.A. “Role of Nitrogen for Plant Growth and Development: A Review”. Advances in Environmental Biology, 10, 209-219, (2016).
[8]. Masaaki Hosomi. and Ryuichi Sudo. “Simultaneous determination of total nitrogen and total phosphorus in freshwater samples using persulfate digestion”, International Journal of Environmental Studies, 27:267-275, (1986). DOI: https://doi.org/10.1080/00207238608710296
[9]. Shah Jahan & Wahocho, Niaz & Laghari, Ghulam & Laghari, Abdul & Bhabhan, Ghulam & HussainTalpur, Khalid & Ahmed, Tofique & Wahocho, Safdar & Lashari, Ayaz. “Role of Nitrogen for Plant Growth and Development: A review”. Advances in Environmental Biology. 10. 209-218, (2016).
[10]. “Standard Methods for the Examination of Water and Wastewater”, 23rd ed.; American Public Health Association: Washington, D.C., (2017).
[11]. T. L. Provin and J. L. Pitt. “Phosphorus--Too Much and Plants May Suffer”, Texas Agricultural Extension Service, The Texas A&M University System, (2008)..
[12]. VietNam Standards 7373:2004, “Soils quality – Index values of total Nitrogen content in the soils of Vietnam”, (2004).
[13]. VietNam Standards 7374:2004, “Soils quality - Index values of total phosphorus content in the soils of Vietnam”, (2004).
[14]. US.EPA. “Method 353.2, Determination of Nitrate-Nitrite Nitrogen by Automated Colorimetry”, Revision 2.0, edited by James W. O’Dell, (1993).
[15]. U.S. EPA. “Method 350.1: Nitrogen, Ammonia (Colorimetric, Automated Phenate”, Revision 2.0, edited by James W. O’Dell, (1993).
[16]. U.S. EPA. “Method 351.2, Determination of Total Kjeldahl Nitrogen by Semi-Automated Colorimetry”, Revision 2.0 edited by James W. O’Dell, (1993).
[17]. U.S. EPA. “Method 365.4: “Phosphorous, Total (Colorimetric, Automated, Block Digester AA II)”, (1974).
[18]. U.S. EPA. “Method 300.1: Determination of Inorganic Anions in Drinking Water by Ion Chromatography,” Revision 1.0. Cincinnati, OH, (1997).
[19]. Vietnam Standards 4046-85: Soil - Method of sampling.
[20]. Vietnam Standards 7538-2:2002: Soil quality – Sampling; Part 2: Guide to sampling techniques.
[21]. Waters corporation, “Waters IC-Pak Column and Guard Column”, 091064TP, Revision 0, (1994).
