The extraction procedure of a pluthiophenol-rich extract from the leaves of Pluchea indica L. collected in Hanoi
3 viewsDOI:
https://doi.org/10.54939/1859-1043.j.mst.104.2025.103-111Keywords:
Pluthiophenol; Extraction; Pluchea indica.Abstract
In this study, we evaluated some conditions affecting the extraction of pluthiophenol rich in pluthiophenol from the leaves of Pluchea indica collected in Hanoi. The investigated factors included extraction method, extraction solvent, medicinal plant size, material mass/solvent volume ratio, extraction time, and number of extraction times. Additionally, several methods of refining and enriching pluthiophenol in the Pluchea indica extract were also investigated. The criteria used to evaluate and select extraction conditions was the pluthiophenol content by the HPLC method. The best results included using hot extraction at 80 oC, extraction solvent of 60% ethanol, material size of 1 mm, extraction three times and 2 hours each time, with material/solvent ratios of 1/8, 1/6, and 1/6 (g/mL). Refining and enriching the pluthiophenol component was done using a liquid-liquid distribution and column chromatography (Amberlite XAD4 polymer adsorbent). The obtained extract after refining had a pluthiophenol content of 6.12%, and the pluthiophenol recovery efficiency of the whole process was 79.74% on a laboratory scale (50 g). In the 1 kg scale extraction procedure, the obtained extract had a pluthiophenol content of 6.29%.
References
[1]. Sabrin R. M. Ibrahim, Alaa A. Bagalagel, Reem M. Diri, Ahmad O. Noor, Hussain T. Bakhsh, Gamal A. Mohamed, “Phytoconstituents and pharmacological activities of indian camphorweed (Pluchea in-dica): A multi-potential medicinal plant of nutritional and ethnomedicinal importance”, Mole-cules, 27(8), 2383, (2022). DOI: https://doi.org/10.3390/molecules27082383
[2]. Jingya Ruan, Zheng Li, Jiejing Yan, Peijian Huang, Haiyang Yu, Lifeng Han, Yi Zhang, Tao Wang, “Bioactive Constituents from the Aerial Parts of Pluchea indica Less”, Molecules, 23 (9), 2104, (2018). DOI: https://doi.org/10.3390/molecules23092104
[3]. Vu Minh Trang, Tran Hoang Anh., Phan Minh Giang, Do Thi Viet Huong, “Some chemical contituents and cytotoxic activity of a thiophene from Pluchea indica in Vietnam”, Vietnam Journal of Chemistry and Applications, 3B(71), p. 219-222, (2024).
[4]. Vu Minh Trang, “Development and validation of a method for quantifying pluthiophenol in Pluchea indica.”, Vietnam Journal of Chemistry and Applications, 02 (75), p. 80-85, (2025).
[5]. Evan H. Crook, Roger P. McDonnell, James T. McNulty, “Removal and Recovery of phenols from in-dustrial waste effluents with amberlite XAD Polymeric adsorbents”, Industrial & Engineering Chem-istry Product Research and Development, 14 (2), p. 113-118, (1975). DOI: https://doi.org/10.1021/i360054a012
